An effective method for extracting total RNA from Dioscorea opposita Thunb.
نویسندگان
چکیده
Dioscorea opposita Thunb., included in the genus Dioscorea of the family Dioscoreaceae, is an important herb with great edible and medicinal value. In this study, the total RNA from leaves of Lichuan Dioscorea opposita Thunb. was isolated by an improved Trizol method. The results showed that the RNA extracted by the improved Trizol method had good integrity, and the RNA could be used for down-stream molecular biology operations including reverse transcription-polymerase chain reaction.
منابع مشابه
Extraction of total DNA and optimization of the RAPD reaction system in Dioscorea opposita Thunb.
Dioscorea opposita Thunb. has been used as health food and herbal medicinal ingredients in traditional Chinese medicine. In this study, the total DNA of D. opposita Thunb. was extracted using an improved cetyltrimethylammonium bromide (CTAB) method, and the extracted DNA was further used for random amplified polymorphic DNA (RAPD) reaction system by design of the L16 (4(4)) orthogonal diagram. ...
متن کاملCarpathian Journal of Food Science and Technology
Article history: Received: 14 February 2016 Accepted in revised form: 20 March 2016 ABSTRACT In recent years, Dioscorea opposita shares a bigger market as people pay more and more attention to health. Dioscorea opposita Thunb. cv. Tiegun is deeply favored by people as it has high edible value, medicinal value and nutritional value. Relatively concentrated harvest season of Dioscorea opposita Th...
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The present study was designed to determine the physicochemical properties of yam (Dioscorea) solution fermented by L. bulgaricus, the protective effect of fermented Dioscorea opposita Thunb. on stomach lesions, and the possibility of its application to fermented dairy products. When the concentration of yam increased, the pH increased in raw powder samples but not in extracted powder samples. ...
متن کاملFunctional properties of dioscorin, a soluble viscous protein from Japanese yam (Dioscorea opposita thunb.) tuber mucilage Tororo.
A soluble viscous protein was purified from yam (Dioscorea opposita Thunb.) tuber mucilage tororo by chromatographic steps, and its functional properties were estimated. The purified dioscorin having the molecular weight of about 200 kDa exhibited high scavenging activities against hydroxyl radicals (IC50 = 195.1 microg/ml) and superoxide anion radicals (IC50 = 92.7 microg/ml). Moreover, it sho...
متن کاملCharacterization and Antihyperglycemic Activity of a Polysaccharide from Dioscorea opposita Thunb Roots
A polysaccharide DOTP-80 from Dioscorea opposita Thunb was obtained by using the method of acid water-extraction and ethanol-precipitation. After being purified by chromatography, the structure characteristics of DOTP-80 were established. Based on the calibration curve obtained with standard dextrans, the molecular weight of the polysaccharide fraction DOTP-80 was calculated to be 123 kDa. The ...
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ورودعنوان ژورنال:
- Genetics and molecular research : GMR
دوره 13 1 شماره
صفحات -
تاریخ انتشار 2014